rabbit anti gdf15 igg  (Bioss)

Journal: Journal of experimental & clinical cancer research : CR

Article Title: Growth differentiation factor 15 contributes to cancer-associated fibroblasts-mediated chemo-protection of AML cells.

doi: 10.1186/s13046-016-0405-0

Figure Lengend Snippet: Fig. 4 GDF15 contributed to the CAF-mediated chemo-protection of the AML cells. (a-c) Western blot, ELISA and Quantitative RT-PCR analyses represented GDF15 expression in the MSC and the CAF cells. d The bar plots showed the relative viability of the THP-1 cells cultured in medium alone or co-cultured with CAFs added with or without an anti-GDF15 antibody under the treatment of Ara-c (10uM) for 48 h. e Quantitative RT- PCR showing GDF15 expression in the CAFs after nucleofection of a GDF15-specific siRNA or a non-targeting control siRNA for 48 h. f The number of viable THP-1 cells cultured in medium alone or direct co-cultured with the layer of CAFs after nucleofection of GDF15-siRNA or not under the treatment of Ara-C (10uM) for 48 h. The data are the result of three independent experiments and are presented as means ± SD,*p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: The rabbit anti-FSP1/S100A4 monoclonal antibody (Millipore, Billerica, MA, USA), rabbit anti-FAP polyclonal antibody (Abnova, Taipei, Taiwan), goat anti-α-SMA polyclonal antibody (Abcam, Cambridge, MA, USA), rabbit anti-GDF15 monoclonal antibody (Cell Signaling, Danvers, MA, USA), mouse antiVimentin monoclonal antibody (Santa Cruz, Dallas, CA, USA), and mouse anti-CD68 monoclonal antibody (Abcam) incubations were performed according to the manufacturer’s instructions.

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Expressing, Cell Culture, Control

Journal: Journal of experimental & clinical cancer research : CR

Article Title: Growth differentiation factor 15 contributes to cancer-associated fibroblasts-mediated chemo-protection of AML cells.

doi: 10.1186/s13046-016-0405-0

Figure Lengend Snippet: Fig. 5 GDF15 expressed in the BM of AML in vivo. a ELISA analysis of GDF15 levels in the BM aspirates of the controls (n = 11) and treatment- naïve AML-CR (n = 9) and AML-refractory patients (n = 8) *** p < 0.001vs the control group. b Examples of immunohistochemistry for GDF15 in the BM sections of the normal control and the primary AML patient. Both images are at a magnification of 400×. c Immunohistochemistry identifies cells that express FAP (arrows) and GDF15 (arrowheads) in the AML patients. GDF15 could also be detected in the leukemia cells (triangle). The images on the left are at a magnification of 400× and on the right are at a magnification of 1000×

Article Snippet: The rabbit anti-FSP1/S100A4 monoclonal antibody (Millipore, Billerica, MA, USA), rabbit anti-FAP polyclonal antibody (Abnova, Taipei, Taiwan), goat anti-α-SMA polyclonal antibody (Abcam, Cambridge, MA, USA), rabbit anti-GDF15 monoclonal antibody (Cell Signaling, Danvers, MA, USA), mouse antiVimentin monoclonal antibody (Santa Cruz, Dallas, CA, USA), and mouse anti-CD68 monoclonal antibody (Abcam) incubations were performed according to the manufacturer’s instructions.

Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Control, Immunohistochemistry

Journal: Scientific Reports

Article Title: Combined Secretomics and Transcriptomics Revealed Cancer-Derived GDF15 is Involved in Diffuse-Type Gastric Cancer Progression and Fibroblast Activation

doi: 10.1038/srep21681

Figure Lengend Snippet: 15 DGC related genes.

Article Snippet: The membranes were blocked with PVDF Blocking Reagent for Can Get Signal (TOYOBO, Osaka, Japan) for 1 h. Anti-GDF15 rabbit polyclonal antibody (L300; Cell Signaling Technology, MA, USA) diluted 1:1000 and anti-β-actin goat polyclonal antibody (C-11; Santa Cruz Biotechnology, TX, USA) diluted 1:500 were used as primary antibodies.

Techniques:

Journal: Scientific Reports

Article Title: Combined Secretomics and Transcriptomics Revealed Cancer-Derived GDF15 is Involved in Diffuse-Type Gastric Cancer Progression and Fibroblast Activation

doi: 10.1038/srep21681

Figure Lengend Snippet: ( A ) GDF15 protein expression of the six gastric cancer cell lines. KATO-III, OCUM-1, NUGC-4, and MKN-45 belonged to the diffuse-type and MKN-7 and MKN-74 represented the intestinal-type. ( B ) GDF15 protein expression of gastric cancer tissues. Left , gastritis (benign); center , intestinal-type gastric cancer (malignant); right , diffuse-type gastric cancer (signet ring cell carcinoma, malignant). Bars indicate 100 μm.

Article Snippet: The membranes were blocked with PVDF Blocking Reagent for Can Get Signal (TOYOBO, Osaka, Japan) for 1 h. Anti-GDF15 rabbit polyclonal antibody (L300; Cell Signaling Technology, MA, USA) diluted 1:1000 and anti-β-actin goat polyclonal antibody (C-11; Santa Cruz Biotechnology, TX, USA) diluted 1:500 were used as primary antibodies.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Combined Secretomics and Transcriptomics Revealed Cancer-Derived GDF15 is Involved in Diffuse-Type Gastric Cancer Progression and Fibroblast Activation

doi: 10.1038/srep21681

Figure Lengend Snippet: ( A ) Comparison of serum GDF15 levels among healthy volunteers (Normal), chronic gastritis patients (Gastritis), and DGC patients. *P < 0.05, **P < 0.01, by Welch’s T-test. ( B , C ) Subgroup analysis of serum GDF15 levels in DGC patients by wall invasion ( B ) and lymph node metastasis ( C ).

Article Snippet: The membranes were blocked with PVDF Blocking Reagent for Can Get Signal (TOYOBO, Osaka, Japan) for 1 h. Anti-GDF15 rabbit polyclonal antibody (L300; Cell Signaling Technology, MA, USA) diluted 1:1000 and anti-β-actin goat polyclonal antibody (C-11; Santa Cruz Biotechnology, TX, USA) diluted 1:500 were used as primary antibodies.

Techniques: Comparison

Journal: Scientific Reports

Article Title: Combined Secretomics and Transcriptomics Revealed Cancer-Derived GDF15 is Involved in Diffuse-Type Gastric Cancer Progression and Fibroblast Activation

doi: 10.1038/srep21681

Figure Lengend Snippet: ( A ) Cell proliferation of NIH3T3 fibroblasts by BrdU incorporation assay. CTRL , no stimulation; GDF15 , 100 ng mL −1 of GDF15 stimulation; TGF-β , 4 ng mL −1 of TGF-β stimulation. ( B ) Differential analysis of 15,554 genes in NIH3T3 fibroblasts between CTRL and GDF15 stimulation. In 45 differentially expressed genes, up- and down-regulated genes are highlighted in red (33 genes) or blue circles (12 genes), respectively. ( C ) Cluster analysis of CTRL, GDF15 or TGF-β stimulation using 45 differentially expressed genes. ( D ) Gene ontology profiles of differentially expressed extracellular genes. Up- and down-regulated genes are highlighted in red or blue, respectively. ( E ) Gene expression enhancement with GDF15 dose dependency of nine extracellular matrix genes. *P < 0.05, by Jonckheere–Terpstra test. Error bars represent standard deviations of three samples.

Article Snippet: The membranes were blocked with PVDF Blocking Reagent for Can Get Signal (TOYOBO, Osaka, Japan) for 1 h. Anti-GDF15 rabbit polyclonal antibody (L300; Cell Signaling Technology, MA, USA) diluted 1:1000 and anti-β-actin goat polyclonal antibody (C-11; Santa Cruz Biotechnology, TX, USA) diluted 1:500 were used as primary antibodies.

Techniques: BrdU Incorporation Assay, Gene Expression

Journal: Scientific Reports

Article Title: Combined Secretomics and Transcriptomics Revealed Cancer-Derived GDF15 is Involved in Diffuse-Type Gastric Cancer Progression and Fibroblast Activation

doi: 10.1038/srep21681

Figure Lengend Snippet: DGC cells secrete GDF15 proteins into the cancer stroma, followed by stromal fibroblast activation. Activated fibroblasts increase the proliferative capacity and express abundant ECM proteins and growth factors. Secreted ECM proteins and growth factors, such as ASPN, POSTN and CTGF, promote the proliferation and invasion of DGC cells.

Article Snippet: The membranes were blocked with PVDF Blocking Reagent for Can Get Signal (TOYOBO, Osaka, Japan) for 1 h. Anti-GDF15 rabbit polyclonal antibody (L300; Cell Signaling Technology, MA, USA) diluted 1:1000 and anti-β-actin goat polyclonal antibody (C-11; Santa Cruz Biotechnology, TX, USA) diluted 1:500 were used as primary antibodies.

Techniques: Activation Assay